Hurma

Kolon Kanseri Tedavisinde Hurmanın Faydaları

13 Mar

Bitki bazlı gıdaların alımı ile kolorektal kanser prevalansındaki bir azalma arasında bildirilen ters ilişki kısmen çözünmez lif ve (poli) fenoller ile bağırsak mikrobiyotası arasındaki etkileşimlerden kaynaklanabilir. Bu çalışmada, randomize, kontrollü, çapraz geçişli bir insan müdahalesi çalışmasında, hem polifenoller hem de lif bakımından zengin olan hurma tarih tüketiminin, kolonik mikrobiyota ve kolon kanseri risk belirteçlerinin büyümesi üzerindeki etkisini değerlendirdik. Toplam 20 sağlıklı insan gönüllüsü rastgele bir kontrol grubuna (maltodekstrin-dekstroz, 37.1 gr) veya bir müdahale grubuna (yedi tarih, yaklaşık 50 gr) atandı. Her bir kol 21 gün sürdü ve çaprazlama şeklinde 14-d arınma periyodu ile ayrıldı. Mikrobiyota gelişimindeki değişiklikler floresan in situ hibridizasyon analizi ile değerlendirilirken, SCFA seviyeleri HPLC kullanılarak değerlendirildi. Ayrıca, amonyak konsantrasyonları, fekal su genotoksisitesi ve anti-proliferasyon kabiliyeti ayrıca hücre çalışması ve Comet testini içeren farklı analizler kullanılarak değerlendirildi. Buna göre diyet alımları, antropometrik ölçümler ve bağırsak hareketi değerlendirmesi de yapıldı. Her ne kadar tarih tüketimi, seçilen bakteri gruplarının veya SCFA’nın büyümesinde önemli değişikliklere neden olmamasına rağmen, barsak hareketlerinde ve dışkı sıklığında ( P <0 · 01; n 21) barsak hareketlerinde ve dışkı sıklığında önemli artışlar ( P < 0 · 05; n 21) bazal değere göre tarih tüketiminden sonra. Dahası, tarih meyve alımı, insan dışkı suyunda kontrole göre genotoksisiteyi önemli ölçüde azalttı ( P <0: 01; n 21). Verilerimiz, meyve tarihinin tüketilmesinin mikrobiyotada değişiklik yapmadan kolon kanseri riskini azaltabileceğini göstermektedir.

Makalenin Orjinal Hali

Impact of palm date consumption on microbiota growth and large intestinal health: a randomised, controlled, cross-over, human intervention study

Abstract

The reported inverse association between the intake of plant-based foods and a reduction in the prevalence of colorectal cancer may be partly mediated by interactions between insoluble fibre and (poly)phenols and the intestinal microbiota. In the present study, we assessed the impact of palm date consumption, rich in both polyphenols and fibre, on the growth of colonic microbiota and markers of colon cancer risk in a randomised, controlled, cross-over human intervention study. A total of twenty-two healthy human volunteers were randomly assigned to either a control group (maltodextrin–dextrose, 37·1 g) or an intervention group (seven dates, approximately 50 g). Each arm was of 21 d duration and was separated by a 14-d washout period in a cross-over manner. Changes in the growth of microbiota were assessed by fluorescence in situ hybridisation analysis, whereas SCFA levels were assessed using HPLC. Further, ammonia concentrations, faecal water genotoxicity and anti-proliferation ability were also assessed using different assays, which included cell work and the Comet assay. Accordingly, dietary intakes, anthropometric measurements and bowel movement assessment were also carried out. Although the consumption of dates did not induce significant changes in the growth of select bacterial groups or SCFA, there were significant increases in bowel movements and stool frequency (P<0·01; n 21) and significant reductions in stool ammonia concentration (P<0·05; n21) after consumption of dates, relative to baseline. Furthermore, date fruit intake significantly reduced genotoxicity in human faecal water relative to control (P<0·01; n 21). Our data indicate that consumption of date fruit may reduce colon cancer risk without inducing changes in the microbiota.

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COPYRIGHT: © The Authors 2015

Corresponding author

* Corresponding author: Professor J. P. E. Spencer, fax +44 118 931 0080, email j.p.e.spencer@reading.ac.uk

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